Overview
The WVU Flow Cytometry & Single Cell Core Facility provides instrumentation and scientific support for single cell analysis and sorting. The facility routinely performs analysis of cells for expression of intracellular and extracellular proteins, cell cycle quantification, cytokine production, and cell sorting based on expression of cell surface antigen(s) and/or expression of genetically engineered intracellular
fluorescent proteins. The core is also equipped to support single cell DNA/RNA analysis.
Equipment
- BD FACSAria III
- 10X Genomic Chromium Controller
- BD LSRFortessa
- 10X Genomics Visium CytAssist
- Cytek Aurora
- Miltenyi Biotec GentleMACS Otto Dissociator
- Luminex Guava EasyCyte HT System
- Miltenyi Biotec AutoMACS Pro Separator
- NanoSight NS300
- Miltenyi Biotec MultiMACS Cell24 Separator
- Zetasizer Nano Z
- Meso Scale Discovery QuickPlex SQ120
Contacts
Director
Kathy Brundage, PhD | kbrundage@hsc.wvu.edu | (304) 293-6273
Lab Assistant
Raven Forshee | raven.forshee@hsc.wvu.edu | (304) 293-6273
Acknowledgements
Please acknowledge the WVU Flow Cytometry & Single Cell Core Facility when reporting data generated in the facility. Please include the core name and core ID in the methods and/or acknowledgement section:
WVU Flow Cytometry & Single Cell Core Facility, RRID:SCR_017738 Experiments were performed in the West Virginia University Flow Cytometry & Single Cell Core Facility, which is supported by the Institutional Development Awards (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant numbers U54GM104942(CTR), P20GM121322 (TME CoBRE) and P20GM103434 (WV-INBRE).
If you make use of the following equipment, please also include these funding sources in your acknowledgement:
- LSRFortessa: S10OD016165
- Cytek Aurora: S10OD028605
